Fullmer C S
Anal Biochem. 1984 Nov 1;142(2):336-9. doi: 10.1016/0003-2697(84)90473-1.
A method is described for the facile identification of half-cystine residues in peptides from protein digests. Combined use of S-beta-(4-pyridylethylation) of cysteine residues with 4-vinyl pyridine and peptide mapping by reversed-phase high-performance liquid chromatography provides the basis for this procedure. Essentially simultaneous, in-line effluent monitoring at 220, 280, and 254 nm provides highly sensitive, specific, and instantaneous identification of all peptide peaks, as well as those containing tryptophan and half-cystine residues, respectively. The resultant cysteine-derivatized peptides are well suited to amino acid compositional and protein sequence analysis, and are protected from loss during enzymatic digestion and/or peptide mapping.
描述了一种用于轻松鉴定蛋白质消化产物中肽段半胱氨酸残基的方法。半胱氨酸残基与4-乙烯基吡啶进行S-β-(4-吡啶基乙基化)，并通过反相高效液相色谱进行肽图谱分析，二者结合为该方法提供了基础。在220、280和254nm处基本同时进行在线流出物监测，可对所有肽峰以及分别含有色氨酸和半胱氨酸残基的肽峰进行高度灵敏、特异且即时的鉴定。所得的半胱氨酸衍生化肽段非常适合进行氨基酸组成分析和蛋白质序列分析，并且在酶促消化和/或肽图谱分析过程中能防止损失。
