从嗜碱真菌中分离并鉴定出一种具有抗真菌活性的新型疏水蛋白Sa-HFB1。
Isolation and Characterization of a Novel Hydrophobin, Sa-HFB1, with Antifungal Activity from an Alkaliphilic Fungus, .
作者信息
Kuvarina Anastasia E, Rogozhin Eugene A, Sykonnikov Maxim A, Timofeeva Alla V, Serebryakova Marina V, Fedorova Natalia V, Kokaeva Lyudmila Y, Efimenko Tatiana A, Georgieva Marina L, Sadykova Vera S
机构信息
Laboratory of Taxonomic Study and Collection of Cultures of Microorganisms, Gause Institute of New Antibiotics, St. Bolshaya Pirogovskaya, 11, 119021 Moscow, Russia.
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, RAS, St. Miklukho-Maklaya, 16/10, 117997 Moscow, Russia.
出版信息
J Fungi (Basel). 2022 Jun 23;8(7):659. doi: 10.3390/jof8070659.
The adaptations that alkaliphilic microorganisms have developed due to their extreme habitats promote the production of active natural compounds with the potential to control microorganisms, causing infections associated with healthcare. The primary purpose of this study was to isolate and identify a hydrophobin, Sa-HFB1, from an alkaliphilic fungus, . A potential antifungal effect against pathogenic and opportunistic fungi strains was determined. The MICs of Sa-HFB1 against opportunistic and clinical fungi ranged from 1 to 8 µg/mL and confirmed its higher activity against both non- and clinical isolates. The highest level of antifungal activity (MIC 1 µg/mL) was demonstrated for the clinical isolate 297 m. The hydrophobin Sa-HFB1 may be partly responsible for the reported antifungal activity of , and may serve as a potential source of lead compounds, meaning that it can be developed as an antifungal drug candidate.
嗜碱微生物因其极端生境而形成的适应性促进了具有控制微生物潜力的活性天然化合物的产生，这些微生物会引发与医疗保健相关的感染。本研究的主要目的是从一种嗜碱真菌中分离并鉴定一种 hydrophobin，即 Sa-HFB1。测定了其对致病性和机会性真菌菌株的潜在抗真菌作用。Sa-HFB1 对机会性真菌和临床真菌的最低抑菌浓度（MIC）范围为 1 至 8 µg/mL，并证实其对非临床分离株和临床分离株均具有更高的活性。临床分离株 297 m 表现出最高水平的抗真菌活性（MIC 为 1 µg/mL）。 hydrophobin Sa-HFB1 可能部分解释了所报道的该真菌的抗真菌活性，并且可能作为先导化合物的潜在来源，这意味着它可以被开发成为一种抗真菌药物候选物。
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