Isolation and purification of bovine immunoglobulins: use of Sephacryl S-300 filtration avoids protein precipitation steps.
作者信息
Collard A, Pivont P, Portetelle D, Gregoire R, Burny A, Antoine H
出版信息
Ann Rech Vet. 1984;15(4):497-501.
PMID:6529119
Abstract
A simplified purification method for bovine IgG1, IgG2, IgM, IgA and S-IgA is presented. It takes advantage of Sephacryl S-300 gel filtration. When applied to this column, colostral whey can be separated into IgM, S-IgA and IgG rich fractions. Filtration of blood serum leads to IgM, IgA and IgG rich fractions. IgM, IgG1, IgG2, S-IgA and IgA are then further purified by anion exchange chromatography.
摘要
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