Stone S S, Jensen M T, Kemeny L J, Wiltsey L
J Immunol Methods. 1976;11(3-4):333-43. doi: 10.1016/0022-1759(76)90127-7.
Similar immunoglobulin (Ig) classes were obtained from porcine colodtral whey by either column or batch chromatographic procedures; a stepwise buffer elution technique was used. Specific transmissible gastroenteritis virus neutralizing antibody was found in the 4 major fractions eluted comprising of IgG1, IgG2, IgA, and IgM. The IgG1, and IgG2 were essentially homogeneous, and the IgA- AND IgM-rich fractions had to be recycled several times through Sephadex G-200 to obtain pure IgA and IgM that had specific virus neutralizing activities per mg of protein of 342.1 and 302. 4, compared with 7.6 for IgG. By a combination of the batch chromatographic procedures and gel filtration, gram amounts of specific Ig could be fractionated from the same colostrum.
通过柱色谱或批量色谱法从猪初乳乳清中获得了相似的免疫球蛋白（Ig）类别；采用了逐步缓冲液洗脱技术。在洗脱的4个主要组分中发现了特异性的传染性胃肠炎病毒中和抗体，这些组分包括IgG1、IgG2、IgA和IgM。IgG1和IgG2基本均一，富含IgA和IgM的组分必须通过Sephadex G - 200多次循环，以获得每毫克蛋白质具有342.1和302.4特异性病毒中和活性的纯IgA和IgM，相比之下IgG的该活性为7.6。通过批量色谱法和凝胶过滤相结合，可以从同一初乳中分离出克级量的特异性Ig。
