Antonova M, Ivanov S, Gavazova E, Chelibonova-Lorer H
Int J Biochem. 1984;16(12):1313-9. doi: 10.1016/0020-711x(84)90234-9.
Comparative studies on fucoprotein metabolism of chicken liver and hepatoma Mc-29 have been carried out and the following parameters were determined: the incorporation rate of [14C]fucose into hepatoma and liver total tissue homogenate, acid-soluble and acid-insoluble fractions, acid-soluble nucleotide fraction and into plasma-membrane acid-precipitable fraction; the activity of microsomal and plasma-membrane fucosyltransferase; the electrophoretic pattern of hepatoma and liver plasma-membrane proteins and the incorporation of [14C]fucose into the glycoprotein fractions in both plasma-membrane preparations. It was found that the labelling of hepatoma tissue homogenate and plasma membranes was higher than that of the same liver preparations 3 hr after the [14C]fucose injection. This finding was supported by a considerably elevated hepatoma fucosyltransferase activity. The labelling rate of numerous fucoproteins from hepatoma plasma membranes was greatly increased and some of the individual glycoprotein bands were labelled to a higher extent compared with liver. The data presented show specific alterations of fucose and fucoprotein metabolism which could be considered as a characteristic feature of chicken viral-induced hepatoma Mc-29.
已对鸡肝脏和肝癌Mc-29的岩藻糖蛋白代谢进行了比较研究，并测定了以下参数：[14C]岩藻糖掺入肝癌和肝脏总组织匀浆、酸溶性和酸不溶性部分、酸溶性核苷酸部分以及质膜酸沉淀部分的掺入率；微粒体和质膜岩藻糖基转移酶的活性；肝癌和肝脏质膜蛋白的电泳图谱以及[14C]岩藻糖在两种质膜制剂糖蛋白部分中的掺入情况。结果发现，在注射[14C]岩藻糖3小时后，肝癌组织匀浆和质膜的标记率高于相同肝脏制剂。肝癌岩藻糖基转移酶活性显著升高支持了这一发现。与肝脏相比，肝癌质膜中许多岩藻糖蛋白的标记率大大增加，一些单个糖蛋白条带的标记程度更高。所呈现的数据显示了岩藻糖和岩藻糖蛋白代谢的特定改变，这可被视为鸡病毒性诱导肝癌Mc-29的一个特征。
