Danielpour D, Sirbasku D A
In Vitro. 1984 Dec;20(12):975-80. doi: 10.1007/BF02619670.
In this report, we present evidence that estrogen-dependent rat mammary tumor cells produce autostimulatory (autocrine) growth factors in response to estrogens, and that hormone autonomous cells produce autocrine activities that are no longer under estrogen regulation. Confirmation of the in vivo significance of autostimulatory factors was sought by inoculating rats with mixed populations of estrogen-dependent, responsive, and autonomous MTW9/PL mammary tumor cells, and showing that after 16 weeks growth in castrated male rats, the estrogen-dependent and responsive mammary tumor cells not only survived, but continued to grow. Three out of seven of the clones derived from apparently autonomous tumors were shown to be estrogen-dependent or responsive. These are the first data establishing that estrogen-dependent (responsive) mammary tumor cells are able to grow in the absence of physiological concentrations of sex steroid hormones in vivo, and that autocrine factors secreted from adjacent autonomous cells are able to substitute for the steroid hormone requirement.
在本报告中，我们提供证据表明，雌激素依赖的大鼠乳腺肿瘤细胞会响应雌激素产生自刺激（自分泌）生长因子，而激素自主细胞会产生不再受雌激素调节的自分泌活性。通过给大鼠接种雌激素依赖、有反应和自主的MTW9/PL乳腺肿瘤细胞混合群体，并表明在去势雄性大鼠中生长16周后，雌激素依赖和有反应的乳腺肿瘤细胞不仅存活，而且继续生长，来寻求对自刺激因子体内重要性的证实。从明显自主的肿瘤中获得的七个克隆中有三个被证明是雌激素依赖或有反应的。这些是首批数据，证实了雌激素依赖（有反应）的乳腺肿瘤细胞能够在体内缺乏生理浓度性类固醇激素的情况下生长，并且相邻自主细胞分泌的自分泌因子能够替代对类固醇激素的需求。
