Roccatello D, Coppo R, Rollino C, Martina G, Piccoli G, Sena L M
Int J Tissue React. 1984;6(6):541-5.
The Fc-receptor function of macrophages and monocytes was studied in 10 healthy subjects and 10 patients (5 in phase of clinical activity) affected by systemic vasculitis with histological evidence of renal involvement. Macrophage function was detected by measuring the clearance in vivo of IgG-sensitized 51Cr-labelled autologous red blood cells (RBC). In vitro immune phagocytosis of monocytes was analysed as a kinetic phenomenon by incubating IgG-coated RBC with nearly pure suspensions of peripheral monocytes. All 5 patients studied in phase of clinical activity showed a defective Fc-receptor function in both the assays, as compared with normal subjects. Since a good correlation was found between the in vitro and the in vivo methods, the use of this non-invasive in vitro procedure is proposed as a substitute for the radioactive in vivo techniques in monitoring the course of the above disease.
在10名健康受试者以及10名患有系统性血管炎且有肾脏受累组织学证据的患者（5名处于临床活动期）中，研究了巨噬细胞和单核细胞的Fc受体功能。通过测量体内IgG致敏的51Cr标记自体红细胞（RBC）的清除率来检测巨噬细胞功能。通过将IgG包被的RBC与外周单核细胞的近乎纯悬液孵育，将单核细胞的体外免疫吞噬作为一种动力学现象进行分析。与正常受试者相比，所有5名处于临床活动期的患者在两种检测中均显示Fc受体功能缺陷。由于在体外和体内方法之间发现了良好的相关性，因此建议使用这种非侵入性体外程序替代放射性体内技术来监测上述疾病的病程。
