Jacobson J, Frenz J, Horváth C
J Chromatogr. 1984 Dec 21;316:53-68. doi: 10.1016/s0021-9673(00)96140-5.
Adsorption behavior on silica-bound hydrocarbonaceous sorbents used in high-performance liquid chromatography was investigated by frontal chromatography and elution on a plateau, and procedures were developed for data collection, analysis and correlation. Isotherms of selected compounds were measured, including several of biological relevance. The relative merits of various dynamic methods of isotherm measurement were compared and illustrated by experimental findings. The frontal analysis technique was determined to be more accurate and convenient than other methods. A miniaturized liquid chromatographic system was constructed to measure isotherms with milligram quantities of material by frontal development. The performance of this apparatus compared favorably with that of equipment which has dimensions usual in high-performance liquid chromatography and requires orders of magnitude greater amounts of a substance for isotherm measurement. Thus the miniaturized system is eminently suited for applications when the substance of interest is in short supply, as is the case with many biological substances. The effects of operating parameters on adsorption behavior were investigated, both to optimize the procedure, and to further explore the fundamental of the process.
采用前沿色谱法和平台洗脱法研究了用于高效液相色谱的硅胶键合含烃吸附剂的吸附行为，并开发了数据收集、分析和关联的程序。测定了选定化合物的等温线，包括几种具有生物学相关性的化合物。比较了各种动态等温线测量方法的相对优点，并通过实验结果进行了说明。结果表明，前沿分析法比其他方法更准确、更方便。构建了一个小型化液相色谱系统，通过前沿展开法用毫克量的物质测量等温线。该仪器的性能与高效液相色谱中常用尺寸的设备相比具有优势，后者测量等温线需要数量级更多的物质。因此，当感兴趣的物质供应短缺时，如许多生物物质的情况，小型化系统非常适合应用。研究了操作参数对吸附行为的影响，以优化程序并进一步探索该过程的基本原理。
