Huang J X, Horváth C
Department of Chemical Engineering, Yale University, New Haven, CT 06520.
J Chromatogr. 1987 Oct 2;406:285-94. doi: 10.1016/s0021-9673(00)94036-6.
Adsorption isotherms of proteins were measured by frontal chromatography in a miniaturized chromatographic system with short, narrow-bore columns. The adsorbents were microparticulate, macroporous siliceous cation exchangers, used in the high-performance liquid chromatography of proteins, and the range of mobile phase compositions was similar to that used in elution chromatography for their separation. Adsorption of protein on the wetted stainless-steel parts of the system was reduced by coating the metal surface with a hydrophilic polymer layer. The adsorption isotherms of proteins were concave down and, in most cases, well represented by the Langmuir model. However, the data could also be fitted with reasonable accuracy to the isotherm equation by Jovanović. Although the siliceous cation exchangers were of different provenance, the isotherms of the proteins were similar. Isotherms measured by the shake-flask method were found to be identical with those obtained by frontal chromatography.
在一个配备短而窄内径柱的小型色谱系统中，通过前沿色谱法测量蛋白质的吸附等温线。吸附剂是用于蛋白质高效液相色谱的微粒大孔硅质阳离子交换剂，流动相组成范围与用于洗脱色谱分离时相似。通过用亲水性聚合物层涂覆金属表面，减少了蛋白质在系统湿润不锈钢部件上的吸附。蛋白质的吸附等温线向下凹，在大多数情况下，能很好地用朗缪尔模型表示。然而，数据也能以合理的精度拟合约万诺维奇等温线方程。尽管硅质阳离子交换剂来源不同，但蛋白质的等温线相似。发现通过摇瓶法测量的等温线与通过前沿色谱法获得的等温线相同。
