Collins C A, Chow C K
J Chromatogr. 1984 Dec 28;317:349-54. doi: 10.1016/s0021-9673(01)91674-7.
A rapid and sensitive high-performance liquid chromatographic method with fluorescence detection has been devised for the analysis of vitamin A (retinol) and its acetate. The method employs a C18 reversed-phase column and methanol as an eluent. The detection of these two compounds is monitored with fluorescence excitation at 348 nm and emission at 470 nm. Detector noise established the lower limit of quantitation at approximately 0.5 ng. Plasma samples were employed to evaluate the accuracy, reproducibility, and applicability of the method. Less than 1 ng of vitamin A in plasma (as low as 1 microliter) can be quantitated by this procedure.
已设计出一种采用荧光检测的快速灵敏的高效液相色谱法，用于分析维生素A（视黄醇）及其醋酸酯。该方法采用C18反相柱，以甲醇作为洗脱剂。通过在348nm处进行荧光激发和在470nm处进行发射来监测这两种化合物的检测。检测器噪声确定了大约0.5ng的定量下限。采用血浆样本评估该方法的准确性、重现性和适用性。通过该程序可对血浆中低于1ng的维生素A（低至1微升）进行定量。
