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Determination of lycopene, alpha- and beta-carotene and retinyl esters in human serum by reversed-phase high performance liquid chromatography.

作者信息

Broich C R, Gerber L E, Erdman J W

出版信息

Lipids. 1983 Mar;18(3):253-8. doi: 10.1007/BF02534557.

DOI:10.1007/BF02534557
PMID:6855484
Abstract

A rapid specific, microdetermination of the major human blood carotenoids by high performance liquid chromatography (HPLC) separation and quantitation at 466 nm is detailed in this paper. Serum retinyl esters can also be quantified utilizing the same separation procedure but detected at 325 nm. One hundred microliters of deproteinated serum were extracted with chloroform and injected on a reverse-phase column. Separation occurred within 16 min for all compounds of interest employing a mobile solvent of MeOH/AcN/CHCl3 (47:47:6). All compounds were quantified at the wavelengths cited by integrated peak areas using retinyl acetate as a daily standard. Analysis of serum from a hypercarotenemic anorexia nervosa patient and a person suffering from hypervitaminosis A are presented as examples of the clinical application of this procedure.

摘要

相似文献

1
Determination of lycopene, alpha- and beta-carotene and retinyl esters in human serum by reversed-phase high performance liquid chromatography.
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2
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本文引用的文献

1
High-performance liquid chromatography of 13-cis-retinoic acid and of endogenous retinol in human plasma.
J Chromatogr. 1980 Sep 12;183(3):346-51. doi: 10.1016/s0378-4347(00)81715-6.
2
An evaluation of trace metals, vitamins, and taste function in anorexia nervosa.
Am J Clin Nutr. 1980 Aug;33(8):1801-8. doi: 10.1093/ajcn/33.8.1801.
3
Separation of long-chain fatty acid esters of retinol by high-performance liquid chromatography.通过高效液相色谱法分离视黄醇的长链脂肪酸酯。
Anal Biochem. 1981 Aug;115(2):324-30. doi: 10.1016/0003-2697(81)90013-0.
4
维生素A补充剂对转基因小鼠和细胞培养物中视紫红质突变体苏氨酸-17→甲硫氨酸和脯氨酸-347→丝氨酸的影响。
Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11933-8. doi: 10.1073/pnas.95.20.11933.
4
Reversed phase HPLC analysis of alpha- and beta-carotene from selected raw and cooked vegetables.对选定的生熟蔬菜中α-胡萝卜素和β-胡萝卜素进行反相高效液相色谱分析。
Plant Foods Hum Nutr. 1988;38(4):333-41. doi: 10.1007/BF01091731.
Comparison of reversed-phase and adsorption modes of high-performance liquid chromatography for the assay of fat soluble vitamins in multivitamin tablets.高效液相色谱反相和吸附模式用于多种维生素片中脂溶性维生素测定的比较
J Chromatogr. 1980 Nov 21;200:300-4. doi: 10.1016/s0021-9673(00)84952-3.
5
Metabolism and nutritional significance of carotenoids.类胡萝卜素的代谢及营养意义
Annu Rev Nutr. 1981;1:351-74. doi: 10.1146/annurev.nu.01.070181.002031.
6
Protracted hypervitaminosis A following long-term, low-level intake.
JAMA. 1982 Mar 5;247(9):1317.
7
Fractionation and quantification of beta carotene and vitamin A derivatives in human serum.人血清中β-胡萝卜素和维生素A衍生物的分离与定量
Clin Chem. 1969 Jun;15(6):479-86.
8
Microdetermination of vitamin A and carotenoids in blood and tissue.
Methods Biochem Anal. 1967;15:1-23. doi: 10.1002/9780470110331.ch1.
9
The hypercarotenemia in anorexia nervosa: a comparison of vitamin A and carotene levels in various forms of menstrual dysfunction and cachexia.
Am J Clin Nutr. 1974 Apr;27(4):362-7. doi: 10.1093/ajcn/27.4.362.
10
Metabolism of retinol-binding protein and vitamin A during hypervitaminosis A in the rat.大鼠维生素A过多症期间视黄醇结合蛋白和维生素A的代谢
J Lipid Res. 1975 May;16(3):180-8.