Walters S M, Westerby B C, Gilvydis D M
J Chromatogr. 1984 Dec 28;317:533-44. doi: 10.1016/s0021-9673(01)91694-2.
High-performance liquid chromatographic (HPLC) separations of eighteen phenylurea pesticides were investigated using both reversed-phase and normal-phase systems. A photoconductivity detector, which responds selectively to ionic products formed via postcolumn UV irradiation of photolabile analytes, was connected in tandem with a UV detector permitting serial dual detection of these compounds. The photoconductivity detector responded selectively to the thirteen halogen and sulfur containing compounds whereas the UV detector responses were of the same order of magnitude for all eighteen compounds at 250 nm. The HPLC-UV-photoconductivity detection system was successfully applied to the determination of chloroxuron in strawberries at the official tolerance level of 0.5 ppm. The tandem detectors combined with a choice of columns and chromatographic modes offers enhanced selectivity for the HPLC analysis of these pesticides as trace contaminants in complex samples.
使用反相和正相系统研究了18种苯基脲类农药的高效液相色谱（HPLC）分离。一个光电导检测器与一个紫外检测器串联连接，该光电导检测器对光不稳定分析物经柱后紫外照射形成的离子产物有选择性响应，从而实现对这些化合物的串联双检测。光电导检测器对13种含卤素和硫的化合物有选择性响应，而在250nm波长下，紫外检测器对所有18种化合物的响应量级相同。HPLC-UV-光电导检测系统成功应用于草莓中绿麦隆的测定，其官方耐受水平为0.5ppm。串联检测器结合柱和色谱模式的选择，为这些作为复杂样品中痕量污染物的农药的HPLC分析提供了更高的选择性。
