Liang D X, Shiga N, Matano O, Goto S
J Chromatogr. 1987 Jan 30;387:385-92. doi: 10.1016/s0021-9673(01)94541-8.
A procedure for high-performance liquid chromatographic (HPLC) determination of four active ingredients of dinocap, 2,4-dinitro-6-octylphenyl crotonate, 2,6-dinitro-4-octylphenyl crotonate, 2,4-dinitro-6-octylphenol and 2,6-dinitro-4-octylphenol, in crops was examined. The four compounds were extracted with acetone and re-extracted into hexane. After clean-up successively by hexane-acetonitrile partition and silica gel column chromatography, the compounds were determined by HPLC with an UV detector (245 nm). The column was Cosmosil 5C18 (ODS, 5 micron) and the eluent was methanol-water-acetic acid (330:70:1). The limit of detection was 0.02 ppm and the recoveries from crops (spiked with 0.5 ppm) were 85-100%. The four compounds were determined simultaneously and the method was shown to be applicable to residue analysis of these compounds in crops sprayed with dinocap.
研究了一种用于高效液相色谱（HPLC）测定作物中敌螨普四种活性成分的方法，这四种成分分别是2,4-二硝基-6-辛基苯基巴豆酸酯、2,6-二硝基-4-辛基苯基巴豆酸酯、2,4-二硝基-6-辛基苯酚和2,6-二硝基-4-辛基苯酚。这四种化合物先用丙酮萃取，再用己烷反萃取。依次经过己烷 - 乙腈分配和硅胶柱色谱净化后，用配备紫外检测器（245 nm）的HPLC测定这些化合物。色谱柱为Cosmosil 5C18（ODS，5微米），洗脱液为甲醇 - 水 - 乙酸（330:70:1）。检测限为0.02 ppm，在添加了0.5 ppm的作物中的回收率为85 - 100%。这四种化合物可同时测定，该方法适用于对敌螨普喷洒作物中这些化合物的残留分析。
