Dubur G Y, Dobretsov G E, Deme A K, Dubure R R, Lapshin E N, Spirin M M
J Biochem Biophys Methods. 1984 Dec;10(3-4):123-34. doi: 10.1016/0165-022x(84)90032-0.
Synthesis and optical properties of the new fluorescent probes - styrylpyridinium derivatives - have been described. Various derivatives of styrylpyridinium, e.g. dimethylamino- (DSP-6 and DSP-12), hydroxy- (HSP-14) and sulphoxy-hydroxy- (SHSP-12) compounds show a long-wave absorption band in ethanol at lambda max abs 486, 402 and 383 nm, respectively. The spectra are sensitive to solvent polarity exhibiting negative solvatochromism by 30-40 nm. Moreover, HSP-14 in aprotic solvents has another absorption band around 600 nm, characteristic of the base. The fluorescence of the probes covers the spectral region 480-650 nm, that of HSP-14 being generated only in the absorption region of the main band (402 nm). The absorption, excitation and fluorescence spectra of these compounds in a liposome suspension have been recorded. The binding of probes to liposomes leads to an increase in fluorescence quantum yield as compared to organic solvents (DSP) and water. Energy transfer in the HSP-14----DSP-12 probe pair has been measured in a liposome suspension. The obtained results suggest that the chemical compounds in question may be useful as fluorescent probes. By varying substituents in the compounds, different spectral characteristics can be attained.
本文描述了新型荧光探针——苯乙烯基吡啶鎓衍生物的合成及其光学性质。苯乙烯基吡啶鎓的各种衍生物，如二甲基氨基 - （DSP - 6和DSP - 12）、羟基 - （HSP - 14）和磺氧基 - 羟基 - （SHSP - 12）化合物，在乙醇中分别在λmax abs为486、402和383 nm处显示长波吸收带。这些光谱对溶剂极性敏感，呈现出30 - 40 nm的负溶剂化显色现象。此外，HSP - 14在非质子溶剂中在600 nm左右有另一个吸收带，这是该碱的特征。探针的荧光覆盖480 - 650 nm光谱区域，其中HSP - 14的荧光仅在主带（402 nm）的吸收区域产生。记录了这些化合物在脂质体悬浮液中的吸收、激发和荧光光谱。与有机溶剂（DSP）和水相比，探针与脂质体的结合导致荧光量子产率增加。在脂质体悬浮液中测量了HSP - 14----DSP - 12探针组中的能量转移。所得结果表明，所讨论的化合物可用作荧光探针。通过改变化合物中的取代基，可以获得不同的光谱特性。
