Aguilera J A, Linares A, Arce V, Garcia-Peregrin E
Int J Biochem. 1983;15(12):1481-4. doi: 10.1016/0020-711x(83)90083-6.
The role of neonatal chick liver and kidneys in the incorporation of mevalonic acid into squalene, lanosterol and cholesterol was studied. Differences between the synthesizing ability of these and other tissues and the influence of the in vivo or in vitro conditions were also examined. In the in vivo experiments, distribution of radioactivity among the nonsaponifiable lipids was not dependent of the doses of mevalonic acid injected. About 80-95% of radioactivity was recovered as cholesterol in liver and brain, whereas in kidneys this percentage was only about 35%. Squalene and lanosterol were formed by kidneys in a high percentage, higher than in liver and other tissues. 12 hr after mevalonate injection, the percentage of cholesterol formed by kidneys increased until more than 50%. In the in vitro experiments carried out in the presence of 0.045-4.0 mM mevalonate, cholesterol was also the main nonsaponifiable identified, but in a lesser percentage than in vivo. In the same conditions, the incorporation of mevalonic acid by kidneys was maximal into squalene. After in vitro incubations for 2 hr, the percentage of cholesterol in kidneys also increased.
研究了新生雏鸡肝脏和肾脏在将甲羟戊酸掺入角鲨烯、羊毛甾醇和胆固醇中的作用。还研究了这些组织与其他组织合成能力的差异以及体内或体外条件的影响。在体内实验中，放射性在非皂化脂质中的分布与注射的甲羟戊酸剂量无关。在肝脏和大脑中，约80-95%的放射性以胆固醇形式回收，而在肾脏中这一比例仅约为35%。肾脏中角鲨烯和羊毛甾醇的形成比例很高，高于肝脏和其他组织。注射甲羟戊酸12小时后，肾脏形成的胆固醇比例增加到50%以上。在存在0.045-4.0 mM甲羟戊酸的体外实验中，胆固醇也是主要鉴定出的非皂化物质，但比例低于体内。在相同条件下，肾脏将甲羟戊酸掺入角鲨烯的量最大。体外孵育2小时后，肾脏中胆固醇的比例也增加。
