Puricelli L, Colombo L L, Bal de Kier Joffé E, de Lustig E S
Invasion Metastasis. 1984;4(4):238-46.
The adhesive and invasive capacities of a transplantable mammary adenocarcinoma that grew spontaneously in a BALB/c mouse with a moderate lung metastatic ability (M3) and a related variant tumor with a higher metastatic potential (MM3) were confronted with precultivated fragments of neonatal syngeneic lung and kidney in three-dimensional culture. M3 cells adhered only to lung as isolated clusters or surrounded the fragments, forming a dense rim. These adherent cells invaded half of the lung pieces in few places and as thick tongues. On the other side, the highly metastatic tumor MM3 adhered to lung and kidney with a similar frequency, mostly surrounding the confronted fragments as a monolayer. MM3 cells deeply infiltrated the lung and kidney pieces, at several points up to 70 and 100%, respectively, either as fine strands or as solitary cells. Invasion by MM3 cells was always accompanied by a dense, homogeneous, acidophilic necrosis of the whole fragments. The higher in vitro invasiveness of MM3 cells could be associated to their higher metastasizing potential in vivo.
将具有中等肺转移能力（M3）的可移植性乳腺腺癌及其具有更高转移潜能的相关变异肿瘤（MM3），在三维培养中与新生同基因肺和肾的预培养片段进行黏附及侵袭能力对比。M3细胞仅以孤立的细胞簇形式黏附于肺，或围绕片段形成致密边缘。这些黏附细胞在少数部位以粗大的舌状侵入肺组织的一半。另一方面，高转移性肿瘤MM3以相似频率黏附于肺和肾，大多作为单层细胞围绕接触的片段。MM3细胞深入浸润肺和肾组织，在多个部位分别高达70%和100%，呈细条索状或单个细胞。MM3细胞的侵袭总是伴随着整个片段致密、均匀的嗜酸性坏死。MM3细胞在体外更高的侵袭性可能与其在体内更高的转移潜能相关。
