Alonso D F, Farías E F, Urtreger A, Ladeda V, Vidal M C, Bal De Kier Joffé E
Institute of Oncology Angel H. Roffo, University of Buenos Aires, Argentina.
J Surg Oncol. 1996 Aug;62(4):288-97. doi: 10.1002/(SICI)1096-9098(199608)62:4<288::AID-JSO14>3.0.CO;2-1.
We characterized a new mammary tumor cell line, F3II, previously established in vitro from a clonal subpopulation of the BALB/c transplantable mammary adenocarcinoma M3, moderately metastatic to lung. The F3II cell line has been passaged > 50 times. It has grown as elongated cells adherent to the bottom of the flask. Cytogenetic studies showed that F3II cultures were nearly triploid. Tumor cells expressed fibronectin and showed high levels of cell-surface urokinase, a key protease in invasion and metastasis. F3II cells grew as poorly differentiated, spindle-cell carcinoma tumors (sarcomatoid carcinomas) with a prominent local invasiveness, a high angiogenic response, and a 90-100% incidence of lung metastases when inoculated s.c. into syngeneic mice. Ultrastructural and immunocytochemical analysis revealed characteristic features of carcinomas. Our data suggest that F3II is less differentiated and more aggressive than the original tumor line, supporting the notion that mammary carcinomas are heterogeneous neoplasms and contain subpopulations with diverse biologic behavior. The F3II mouse mammary sarcomatoid carcinoma line is a suitable model to examine antiinvasive, antiangiogenic, and antimetastatic agents.
我们鉴定了一种新的乳腺肿瘤细胞系F3II，它先前是从BALB/c可移植性乳腺腺癌M3的一个克隆亚群体外建立的，该腺癌向肺的转移能力中等。F3II细胞系已传代超过50次。它以细长细胞的形式生长，贴附于培养瓶底部。细胞遗传学研究表明，F3II培养物接近三倍体。肿瘤细胞表达纤连蛋白，并显示出高水平的细胞表面尿激酶，这是侵袭和转移中的一种关键蛋白酶。F3II细胞生长为低分化的梭形细胞癌肿瘤（肉瘤样癌），具有显著的局部侵袭性、高血管生成反应，当皮下接种到同基因小鼠体内时，肺转移发生率为90 - 100%。超微结构和免疫细胞化学分析揭示了癌的特征性特点。我们的数据表明，F3II比原始肿瘤细胞系分化程度更低且更具侵袭性，支持了乳腺癌是异质性肿瘤且包含具有不同生物学行为的亚群这一观点。F3II小鼠乳腺肉瘤样癌细胞系是用于检测抗侵袭、抗血管生成和抗转移药物的合适模型。
