Müller U
J Embryol Exp Morphol. 1984 Nov;83 Suppl:41-9.
In order to learn more about the role of sex chromosome-dependent gene products in gonadogenesis, changes in protein patterns were studied during gonadal development. Two-dimensional gel electrophoresis analysis revealed specific proteins in both sexes at all developmental stages. Evidently the gonads are not indifferent by biochemical criteria at any developmental stage and express several specific genes from the onset of differentiation. To correlate these polypeptides with the sex chromosomes, proteins were investigated in human-rodent somatic cell hybrids and in genetically identical cell clones differing in one sex chromosome only. On two-dimensional gels one Y-dependent polypeptide was found with similar characteristics (relative molecular mass and isoelectric point) as an early testicular polypeptide. Its identity, however, remains to be proven.
为了更多地了解性染色体依赖性基因产物在性腺发生中的作用，研究了性腺发育过程中蛋白质模式的变化。二维凝胶电泳分析揭示了两性在所有发育阶段都存在特定蛋白质。显然，从生化标准来看，性腺在任何发育阶段都不是中性的，并且从分化开始就表达几种特定基因。为了将这些多肽与性染色体联系起来，在人-啮齿动物体细胞杂种以及仅在一条性染色体上不同的基因相同的细胞克隆中研究了蛋白质。在二维凝胶上发现了一种Y依赖性多肽，其具有与早期睾丸多肽相似的特征（相对分子质量和等电点）。然而，其身份仍有待证实。
