Huxham I M, Beck F
J Embryol Exp Morphol. 1984 Dec;84:203-15.
Fluid from the extraembryonic coelom of 11.5-day rat embryos cultured in 100% rat serum, 100% human serum and 90% human serum supplemented with 10% rat serum between days 9.5 and 11.5 postconception were compared using polyacrylamide gel electrophoresis and crossed immunoelectrophoresis. The protein composition of the exocoelomic fluids differed considerably from one another and from each of their respective culture sera. The majority of proteins in the exocoelom were derived from macromolecular transport but some contribution was made from protein synthesis by the conceptus. Eighteen proteins normally found in rat serum were found in the exocoelom of conceptuses cultured in 100% rat serum. Eighteen proteins were found in the exocoelom of rat conceptuses cultured in 100% human serum, of which ten were derived from human serum and eight were proteins normally found in rat serum. Analysis of fluid from conceptuses cultured in 90% human serum supplemented with 10% rat serum showed eleven human serum proteins and ten rat serum proteins. Differences in the composition of both human and rat proteins between the latter two fluids were also evident.
使用聚丙烯酰胺凝胶电泳和交叉免疫电泳，比较了在受孕后9.5至11.5天期间，在100%大鼠血清、100%人血清以及补充了10%大鼠血清的90%人血清中培养的11.5天大的大鼠胚胎的胚外体腔液。胚外体腔液的蛋白质组成彼此之间以及与各自的培养血清都有很大差异。胚外体腔中的大多数蛋白质来自大分子转运，但也有一些是由孕体的蛋白质合成产生的。在100%大鼠血清中培养的孕体的胚外体腔中发现了18种通常存在于大鼠血清中的蛋白质。在100%人血清中培养的大鼠孕体的胚外体腔中发现了18种蛋白质，其中10种来自人血清，8种是通常存在于大鼠血清中的蛋白质。对在补充了10%大鼠血清的90%人血清中培养的孕体的液体分析显示，有11种人血清蛋白和10种大鼠血清蛋白。后两种液体中人和大鼠蛋白质组成的差异也很明显。
