Gulamhusein A P, Pratten M K, Williams C A, Beck F
Department of Anatomy, University of Leicester, UK.
J Anat. 1990 Feb;168:113-21.
We describe the results of experiments to study the effects on rat conceptuses cultured in human serum supplemented with rat serum which was separated into high (greater than 30 kDa) and low (less than 30 kDa) molecular weight fractions by ultrafiltration. Ultrafiltered rat serum was found to lack certain growth-promoting substances which are necessary for embryonic growth and differentiation during the culture period. Culture in whole human serum or human serum supplemented with low molecular weight fraction (filtrate) results in conceptuses which grow reasonably well but are anaemic, whilst anaemia is relieved by the high molecular weight fraction. Addition of human or rat transferrin (MW 80 kD approx.) to whole human serum alleviates anaemia, an effect observed more distinctly with rat transferrin.
我们描述了一系列实验的结果，这些实验旨在研究在添加了经超滤分离为高分子量（大于30 kDa）和低分子量（小于30 kDa）组分的大鼠血清的人血清中培养大鼠胚胎的影响。研究发现，超滤后的大鼠血清缺乏胚胎在培养期间生长和分化所需的某些生长促进物质。在全人血清或添加了低分子量组分（滤液）的人血清中培养的胚胎生长良好，但出现贫血症状，而高分子量组分可缓解贫血症状。向全人血清中添加人或大鼠转铁蛋白（分子量约80 kD）可缓解贫血症状，大鼠转铁蛋白的这种作用更为明显。
