Obrant K J, Odselius R
Ultrastruct Pathol. 1984;7(2-3):123-31. doi: 10.3109/01913128409141469.
Energy dispersive X-ray microanalysis (EDX) (or electron microprobe analysis) of the relative intensity for calcium in different bone trabeculae from the tibia epiphysis, and in different parts of one and the same trabecula, was performed on 3 patients who had earlier had a fracture of the ipsilateral tibia-diaphysis. The variation in intensity was compared with the histochemical patterns obtained with both the Goldner and the von Kóssa staining techniques for detecting calcium in tissues. Previously reported calcium distribution features, found to be typical for posttraumatic osteopenia, such as striated mineralization patterns in individual trabeculae and large differences in mineralization level between different trabeculae, could be verified both by means of the two histochemical procedures and from the electron microprobe analysis. A pronounced difference was observed, however, between the two histochemical staining techniques as regards their sensitivity to detect calcium. The two methods have different turnover points, from negative to positive along a gradient change of calcium concentrations. To judge from the values obtained from the EDX measurements, the sensitivity of the Goldner technique should be more than ten times higher than that of von Kóssa. The EDX measurements gave more detailed information than either of the two histochemical techniques: great variations in the intensity of the calcium peak were found in trabeculae stained as unmineralized as well as mineralized.
对3例曾有同侧胫骨干骨折的患者，进行了能量色散X射线微分析（EDX）（或电子微探针分析），以测定胫骨骨骺不同骨小梁以及同一骨小梁不同部位钙的相对强度。将强度变化与用戈德纳（Goldner）染色技术和冯·科萨（von Kóssa）染色技术检测组织中钙所获得的组织化学模式进行比较。先前报道的创伤后骨质减少典型的钙分布特征，如单个骨小梁中的条纹状矿化模式以及不同骨小梁之间矿化水平的巨大差异，可通过这两种组织化学方法以及电子微探针分析得到证实。然而，在检测钙的敏感性方面，观察到两种组织化学染色技术之间存在明显差异。这两种方法有不同的转折点，即随着钙浓度的梯度变化从阴性变为阳性。从EDX测量获得的值判断，戈德纳技术的敏感性应比冯·科萨技术高十多倍。EDX测量提供了比两种组织化学技术中任何一种都更详细的信息：在未矿化和矿化染色的骨小梁中都发现了钙峰强度的巨大变化。
