Malchesky P S, Wojcicki J, Moorman M, Pentermann E J, Lewandowski J, Nose Y
Trans Am Soc Artif Intern Organs. 1984;30:313-9.
The blood cell and membrane properties are important in determining hemolysis and cell deposition, as shown previously, and thus are determinants of the maximum plasma separation rates. In general, membranes with large mean pore sizes exhibit hemolysis at lower operating transmembrane pressures. Surface structure and not necessarily por tortuosity has been found to be more important, at least for the hollow fiber membranes studied. Mean membrane pore data coupled with SEM observations were useful in correlating red cell lysis with membrane properties. The red cell may be likened to a liquid drop, encased by a flexible deformable membrane. Cell deformation, orientation, and rotation increase as does interfacial surface tension with rising rates of shear. A high correlation of interfacial surface tension with shear rate was determined using a filtration model incorporating cell deformability. Interfacial surface tensions of less than 6 dynes/cm for shear rates up to 1000s-1 were found. Inclusion of blood and membrane properties in the analysis of plasma separation gives significantly better fit of the experimental data to theoretical correlations than do other models which exclude their consideration.
如前所示，血细胞和膜特性对于确定溶血和细胞沉积很重要，因此是最大血浆分离速率的决定因素。一般来说，平均孔径较大的膜在较低的操作跨膜压力下会出现溶血现象。至少对于所研究的中空纤维膜而言，已发现表面结构而非孔隙曲折度更为重要。平均膜孔数据与扫描电子显微镜观察结果相结合，有助于将红细胞裂解与膜特性相关联。红细胞可被比作由柔性可变形膜包裹的液滴。随着剪切速率的增加，细胞变形、取向和旋转以及界面表面张力都会增加。使用包含细胞可变形性的过滤模型确定了界面表面张力与剪切速率之间的高度相关性。发现对于高达1000s-1的剪切速率，界面表面张力小于6达因/厘米。在血浆分离分析中纳入血液和膜特性，比其他不考虑这些因素的模型能使实验数据与理论相关性的拟合度显著提高。
