Inouye H, Kirschner D A
J Neurocytol. 1984 Dec;13(6):883-94. doi: 10.1007/BF01148591.
New X-ray reflections have been detected from myelinated tissue of the mammalian C.N.S. Diffraction patterns from unfixed optic nerves of mouse, rat, guinea-pig and rabbit, and unfixed corpus callosum of calf were recorded during X-ray exposures of 1-6 days. The equatorial pattern was typical of lamellar myelin of the C.N.S.; however, the meridional pattern showed new features: a strong, sharp intensity maximum at 30.4 +/- 0.4 A (N = 11) spacing, and a weak, diffuse ring centered at 11.0 +/- 0.5 A (N = 5) spacing. The 30.4 A reflection was neither strictly arced like the equatorial reflections from lamellar myelin nor fully horizontal. Since the 30.4 A meridional reflection was not observed in patterns from myelinated nerve of the P.N.S., we suggest that this diffraction comes from the radial component, or interlamellar tight junctions, which is unique to mammalian C.N.S. myelinated tissue. The diffuse ring at 11.0 A probably comes from myelin protein.
在哺乳动物中枢神经系统的有髓组织中检测到了新的X射线反射。在1至6天的X射线照射期间，记录了小鼠、大鼠、豚鼠和兔子未固定的视神经以及小牛未固定的胼胝体的衍射图样。赤道图样是中枢神经系统板层髓鞘的典型图样；然而，子午线图样显示出了新特征：在间距为30.4 +/- 0.4埃（N = 11）处有一个强烈、尖锐的强度最大值，以及在间距为11.0 +/- 0.5埃（N = 5）处有一个以其为中心的微弱、弥散的环。30.4埃的反射既不像来自板层髓鞘的赤道反射那样严格呈弧形，也不完全是水平的。由于在周围神经系统有髓神经的图样中未观察到30.4埃的子午线反射，我们认为这种衍射来自径向成分或层间紧密连接，这是哺乳动物中枢神经系统有髓组织所特有的。11.0埃处的弥散环可能来自髓鞘蛋白。
