Dermietzel R, Kroczek H
Cell Tissue Res. 1980;213(1):81-94. doi: 10.1007/BF00236922.
The process of myelination in the central nervous system (CNS) of the rat (optic nerve) was studied with the freeze-fracturing technique and ultrathin sectioning to obtain information on the developmental mechanisms of interlamellar tight junctions. Using a tilting cartridge for analysis of thin sections, it could be demonstrated that during the initial phase of wrapping a tight junction formation develops between the joining tips of the oligodendrocytic process. In tannic acid-stained samples these junctions appear as typical quintuple-layered membrane fusions, while in potassium permanganate-stained material membrane thickenings between the apposing glial tips are prevalent. The latter configuration represents the characteristic feature of the so-called radial component of central myelin. Using the freeze-fracturing technique, a biphasic mode of the myelinic tight junction assembly was detected. It is suggested that tight junctions represent a prerequisite of the myelination process.
采用冷冻断裂技术和超薄切片技术，对大鼠中枢神经系统（视神经）的髓鞘形成过程进行了研究，以获取有关层间紧密连接发育机制的信息。使用倾斜载物台对薄片进行分析，可以证明在包裹的初始阶段，少突胶质细胞突起的连接尖端之间会形成紧密连接。在经单宁酸染色的样本中，这些连接表现为典型的五层膜融合，而在经高锰酸钾染色的材料中，相邻胶质细胞尖端之间的膜增厚现象很普遍。后一种结构代表了中枢髓鞘所谓的放射状成分的特征。采用冷冻断裂技术，检测到髓鞘紧密连接组装的双相模式。有人认为紧密连接是髓鞘形成过程的一个先决条件。
