Ruggiero D, Batton R R, Jayaraman A, Carpenter M B
J Comp Neurol. 1977 Mar 15;172(2):189-209. doi: 10.1002/cne.901720202.
Although retrograde and anterograde degeneration studies have provided important information concerning brain stem afferents to the fastigal nucleus (FN), these data may be incomplete and should be confirmed by axonal transport methods. Attempts were made to inject horseradish peroxidase (HRP) unilaterally into the FN in a series of adult cats. Animals were perfused with dextran and a fixative solution of paraformaldehyde and glutaraldehyde in 0.1 M phospate buffer. Representative sections were treated by the Graham and Karnovsky ('66) method. Selective HRP injections in one FN resulted in retrograde transport of the marker to Purkinje cells of the ipsilateral vermis and distinctive appendages of the contralateral medial accessory olivary (MAO) nucleus (nucleus beta and the dorso-medial cell column). Retrograde transport of the label was found bilaterally in cells of the medial (MVN) and inferior (IVN) vestibular nuclei, in cell group x and in the nucleus prepositus (PP). Labeled vestibular neurons, most numerous in MVN, were identified in dorsal, caudal and lateral regions, with a slight ipsilateral preponderance. Only a few neurons in caudal, dorsal and lateral regions of the IVN were labeled and none of these included cells of group f. Labeled cells in the caudal third of PP were greatest ipsilaterally. Rostral and caudal injections of FN labeled smaller numbers of cells in MVN, IVN, cell group x and PP. HRP injections of FN and portions of lobules VIII and IX resulted in bilateral retrograde labeling of larger numbers of cells in MVN, IVN and cell group x, and ipsilateral labeling of cells in group y and the interstitial nucleus of the vestibular nerve. Injections of HRP into basal folia of lobules V and VI resulted in retrograde transport of the marker to cells of the medial and dorsal accessory olivary nuclei contralaterally, and to cells of the ipsilateral accessory cuneate nucleus. Transport of label injected into portions of the pyramis was detected in parts of the contralateral MAO and bilaterally in parts of the pontine and reticulotegmental nuclei. This study suggests that the principal afferents of the fastigial nucleus arise from: (1) Purkinje cells of the ipsilateral vermis, (2) restricted portions of the contralateral MAO (nucleus beta and dorsomedial cell column), (3) portions of the MVN and IVN (bilaterally) and (4) caudal parts of the PP. Secondary vestibular inputs to the fastigial nucleus probably are relayed mainly by Purkinje cells in the cerebellar cortex.
尽管逆行和顺行变性研究已经提供了有关向顶核（FN）的脑干传入神经的重要信息，但这些数据可能并不完整，应该通过轴突运输方法加以证实。我们尝试在一系列成年猫中单侧将辣根过氧化物酶（HRP）注入顶核。动物用葡聚糖以及在0.1M磷酸盐缓冲液中的多聚甲醛和戊二醛固定液进行灌注。代表性切片采用格雷厄姆和卡诺夫斯基（1966年）的方法处理。在一侧顶核进行选择性HRP注射导致标记物逆行运输到同侧小脑蚓部的浦肯野细胞以及对侧内侧副橄榄核（MAO）（β核和背内侧细胞柱）的独特附属结构。在双侧的内侧（MVN）和下（IVN）前庭核、x细胞群和前庭前置核（PP）的细胞中发现了标记物的逆行运输。标记的前庭神经元在MVN中最多，在背侧、尾侧和外侧区域被识别出来，同侧略占优势。IVN尾侧、背侧和外侧区域只有少数神经元被标记，其中不包括f组细胞。PP尾侧三分之一区域的标记细胞同侧最多。顶核的头侧和尾侧注射标记的MVN、IVN、x细胞群和PP中的细胞数量较少。将HRP注入顶核以及小叶VIII和IX的部分区域导致MVN、IVN和x细胞群中大量细胞的双侧逆行标记，以及y组和前庭神经间质核中细胞的同侧标记。将HRP注入小叶V和VI的基底部导致标记物逆行运输到对侧的内侧和背侧副橄榄核的细胞，以及同侧楔束副核的细胞。注入锥体部分的标记物在对侧MAO的部分区域以及脑桥和网状被盖核的部分区域双侧被检测到。这项研究表明，顶核的主要传入神经来自：（1）同侧小脑蚓部的浦肯野细胞，（2）对侧MAO的特定部分（β核和背内侧细胞柱），（3）MVN和IVN的部分区域（双侧），以及（4）PP的尾侧部分。顶核的继发性前庭输入可能主要由小脑皮质中的浦肯野细胞中继。
