Ligeti E, Lukács G L
J Bioenerg Biomembr. 1984 Apr;16(2):101-13. doi: 10.1007/BF00743043.
The membrane potential and calcium accumulation of mitochondria were followed by ion-specific electrodes in the presence of the proton-donor anions phosphate, acetate, glutamate, and beta-hydroxybutyrate. Phosphate was the only anion which allowed rapid and complete restoration of both the membrane potential and the steady-state extramitochondrial calcium concentration after the uptake of 100-200 nmol calcium per mg protein. If there was no influx of any proton-donor anion, the extent of calcium uptake depended on the intramitochondrial phosphate content. Both the fall of the membrane potential and the increase of the external calcium concentration brought about by a given amount of uncoupler were counteracted by phosphate transported into the mitochondria.
在质子供体阴离子磷酸盐、乙酸盐、谷氨酸盐和β-羟基丁酸盐存在的情况下，通过离子特异性电极跟踪线粒体的膜电位和钙积累。磷酸盐是唯一一种在每毫克蛋白质摄取100 - 200纳摩尔钙后能使膜电位和稳态线粒体外钙浓度快速且完全恢复的阴离子。如果没有任何质子供体阴离子的流入，钙摄取的程度取决于线粒体内的磷酸盐含量。由一定量的解偶联剂引起的膜电位下降和外部钙浓度增加，会被转运到线粒体内的磷酸盐抵消。
