Azzone G F, Pozzan T, Massari S
Biochim Biophys Acta. 1978 Feb 9;501(2):307-16. doi: 10.1016/0005-2728(78)90036-1.
The paper reports an analysis of the relationship between deltamuH the proton electrochemical potential difference, and deltaGp, the phosphate potential. Depression of deltamuH and deltaGp has been obtained by titration with: (a) carbonylcyanide trifluoromethoxyphenylhydrazone; (b) nigericin (+ valinomycin); (c) KCl (+ valinomycin); and (d) rotenone. The uncoupler depresses deltamuH more than nigericin (+ valinomycin), KCl (+ valinomycin) and rotenone at equivalent deltaGp. The deltaGp/deltamuH ratio is about 3 at high values of deltamuH. When deltaGp and deltamuH are depressed by nigericin (4 valinomycin) the deltaGp/deltamuH ratio remains constant. When deltaGp and deltamuH are depressed by uncouplers, the deltaGp/deltamuH ratio increases hyperbolically tending to infinity while deltamuH tends to zero. The absence of constant proportionality between deltaGp and deltamuH indicates that the proton gradients driving ATP synthesis presumably operate within microscopic environments.
该论文报道了对质子电化学势差ΔμH与磷酸势ΔGp之间关系的分析。通过用以下物质滴定获得了ΔμH和ΔGp的降低：(a) 羰基氰三氟甲氧基苯腙；(b) 尼日利亚菌素（+缬氨霉素）；(c) 氯化钾（+缬氨霉素）；以及(d) 鱼藤酮。在等效的ΔGp下，解偶联剂比尼日利亚菌素（+缬氨霉素）、氯化钾（+缬氨霉素）和鱼藤酮更能降低ΔμH。在高ΔμH值时，ΔGp/ΔμH比值约为3。当ΔGp和ΔμH被尼日利亚菌素（+缬氨霉素）降低时，ΔGp/ΔμH比值保持恒定。当ΔGp和ΔμH被解偶联剂降低时，ΔGp/ΔμH比值呈双曲线增加，趋向于无穷大，而ΔμH趋向于零。ΔGp和ΔμH之间不存在恒定的比例关系，这表明驱动ATP合成的质子梯度可能在微观环境中起作用。
