Weston K M, Foster R W, Weston A H
J Pharmacol Methods. 1984 Dec;12(4):285-97. doi: 10.1016/0160-5402(84)90014-7.
A technique is described for applying chemical irritants selectively to the skin of a superfused ganglion/body wall preparation of the horse leech, Haemopis sanguisuga. Details are given of the intracellular recording from sensory neurones of the effects of the irritant dibenzoxazepine and of mechanical stimulation. Dibenzoxazepine produced changes in the spontaneous firing pattern in the nociceptive cells of the leech but did not affect the response to mechanical stimulation of the skin in any type of sensory cell. It is possible that the system described could be used as a model of cutaneous excitation evoked by drugs or mechanical stimulation in higher animals.
本文描述了一种将化学刺激物选择性地施加于马蛭（Haemopis sanguisuga）的灌注神经节/体壁制备物皮肤的技术。详细介绍了从感觉神经元进行细胞内记录，观察刺激物二苯并恶唑嗪的作用以及机械刺激的影响。二苯并恶唑嗪使水蛭伤害性感受细胞的自发放电模式发生改变，但不影响任何类型感觉细胞对皮肤机械刺激的反应。所描述的系统有可能被用作高等动物中药物或机械刺激诱发的皮肤兴奋模型。
