Goudie C A
Steroids. 1984 Sep;44(3):241-52. doi: 10.1016/0039-128x(84)90005-9.
A rapid, reproducible extraction procedure was developed to recover the synthetic androgen 17 alpha-methyltestosterone (MT) from fish muscle. A single extraction with chloroform-methanol (2:1 V/V), followed by elution from mini-columns with various methanol concentrations, yielded an extraction efficiency of greater than 70%. Resolution of MT from testosterone was complete and reliable quantitation was achieved utilizing reversed-phase high performance liquid chromatography. Endogenous testosterone was not detectable in 1-g muscle samples, but MT was detected in fish muscle 6 h after adult Tilapia aurea were fed a diet containing the steroid.
开发了一种快速、可重复的提取方法，用于从鱼肉中回收合成雄激素17α-甲基睾酮（MT）。用氯仿-甲醇（2:1 V/V）单次提取，然后用不同甲醇浓度的微型柱洗脱，提取效率大于70%。利用反相高效液相色谱法可完全分离MT和睾酮，并实现可靠定量。在1克肌肉样本中未检测到内源性睾酮，但在成年金色罗非鱼喂食含该类固醇的饲料6小时后，在鱼肉中检测到了MT。
