Sutoo D, Sano K
Alcohol. 1984 Mar-Apr;1(2):141-4. doi: 10.1016/0741-8329(84)90070-3.
The present study was carried out in order to clarify the mechanism of calcium prolongation of ethanol-induced sleep. p-Chlorophenylalanine (PCPA, 300 mg/kg), alpha-methyltyrosine (alpha MPT, 100 mg/kg) and diethyldithiocarbamate (DDC, 250 mg/kg) were administered intraperitoneally (IP) to mice to reduce the levels of serotonin (5-HT), dopamine (DA) and norepinephrine (NE) respectively in the brain. Sleeping time was then measured following the administration of ethanol (4.5 g/kg, IP) both with and without CaCl2 (20 mumol/kg, intravenous (IV)). When saline (IP) plus CaCl2 (IV) was administered, the duration of ethanol-induced sleep was prolonged by 100% as compared with saline (IP) plus saline (IV). Duration of ethanol-induced sleep was not changed by PCPA, alpha MPT and DDC. On the other hand, the prolongation of ethanol-induced sleep by CaCl2 was antagonized by PCPA, alpha MPT and DDC. Also, only the DA level in the cerebrum was increased by 25% by administration of CaCl2. We suggest that the increase in ethanol-induced sleeping time due to CaCl2 results from the increase in biogenic amines in the brain.
本研究旨在阐明钙延长乙醇诱导睡眠的机制。对小鼠腹腔注射对氯苯丙氨酸（PCPA，300mg/kg）、α-甲基酪氨酸（αMPT，100mg/kg）和二乙基二硫代氨基甲酸盐（DDC，250mg/kg），以分别降低脑中5-羟色胺（5-HT）、多巴胺（DA）和去甲肾上腺素（NE）的水平。然后在腹腔注射乙醇（4.5g/kg）的情况下，分别测量注射氯化钙（20μmol/kg，静脉注射）前后的睡眠时间。当腹腔注射生理盐水加静脉注射氯化钙时，与腹腔注射生理盐水加静脉注射生理盐水相比，乙醇诱导的睡眠时间延长了100%。PCPA、αMPT和DDC对乙醇诱导的睡眠时间没有影响。另一方面，PCPA、αMPT和DDC可拮抗氯化钙对乙醇诱导睡眠的延长作用。此外，仅通过注射氯化钙可使大脑中的DA水平升高25%。我们认为，氯化钙导致乙醇诱导睡眠时间增加是由于脑中生物胺增加所致。
