Sano K, Shimojo N, Yamaguchi S
Institute of Community Medicine, University of Tsukuba, Ibaraki.
Nihon Eiseigaku Zasshi. 1990 Jun;45(2):717-22. doi: 10.1265/jjh.45.717.
This study was carried out to clarify the effects of methylmercury intoxication on the ethanol-induced sleeping time of mice. The mice were injected with methylmercury chloride (MMC) (10 mgMMC/kg body weight) subcutaneously for 1, 5 and 10 successive days (1, 5 and 10 inj.), and control mice received only saline. Twenty-four hours, after the last injection, these mice were treated with ethanol (4.5 mgEtOH/kg body weight) intraperitoneally and subsequent sleeping time was observed. After 24 hours, mice were sacrificed to measure the concentration of MMC in various brain regions and liver. A similar experiment with ethanol treatment was also performed to assay the biogenic monoamines in various brain regions and alcohol dehydrogenase (ADH) activity in liver. The results can be summarized as follows: 1) Ethanol-induced sleeping time was 170 min. in the 10 inj. and it was significantly longer than saline, 1 and 5 inj. However, other experimental groups showed no change when compared with saline. 2) Norepinephrine levels increased in white matter and pons + medulla after 1 inj. 3) Dopamine levels increased only in white matter of the 10 inj. when compared with saline. No changes were shown in the other groups. 4) Serotonin levels increased in all the regions after 1 inj. 5) ADH activity in liver did not show any alteration during the experimental period when compared with saline.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究旨在阐明甲基汞中毒对乙醇诱导的小鼠睡眠时间的影响。将小鼠皮下注射氯化甲基汞（MMC）（10 mgMMC/千克体重），连续注射1天、5天和10天（分别为1次、5次和10次注射），对照小鼠仅注射生理盐水。在最后一次注射24小时后，给这些小鼠腹腔注射乙醇（4.5 mgEtOH/千克体重），随后观察睡眠时间。24小时后，处死小鼠以测量不同脑区和肝脏中MMC的浓度。还进行了一项乙醇处理的类似实验，以检测不同脑区的生物源性单胺和肝脏中的乙醇脱氢酶（ADH）活性。结果总结如下：1）在10次注射组中，乙醇诱导的睡眠时间为170分钟，明显长于生理盐水组、1次注射组和5次注射组。然而，与生理盐水组相比，其他实验组没有变化。2）1次注射后，白质和脑桥+延髓中的去甲肾上腺素水平升高。3）与生理盐水组相比，仅在10次注射组的白质中多巴胺水平升高。其他组没有变化。4）1次注射后，所有区域的血清素水平均升高。5）与生理盐水组相比，实验期间肝脏中的ADH活性没有任何改变。（摘要截断于250字）
