Flieger S, Boratyński Z, Welento J, Eustachiewicz R, Szalak M, Krzyzanowski J, Sławomirski J
Pol Arch Weter. 1984;24(2):261-73.
For the studies 4 sexually mature cows were used, in which ovaria and oviducts were cut out bilaterally or unilaterally. The fourth cow was the control of the experiment. The operated animals were kept alive for 21 days and then slaughtered. For studies were taken: the brain stem, spinal cord from the segments Th9 - Th13 and L1 - S4, bilateral spinal ganglions, bilaterally the sympathetic chain from the segment Th10 - S4, and also ganglions and visceral-plexi, anterior mesenteric, intermesenteric, posterior mesenteric, hypogastric and pelvic. The material was fixed in alcohol, embedded in paraffin and cut into 15 micron sections. The sections were stained by Nissel's method. For examinations subsequent sections were taken. Due to the operations performed degeneration changes occurred in nervous cells in the central and peripheral nervous system. They were the basis for determination of the localization of the nervous centres for ovarium and oviduct. The source of preganglionic sympathetic visceromotor fibres for these organs is nucleus intermediolateralis in the segment L2 - L3. The sympathetic postganglionic fibres originate from cells of the sympathetic chain from L1 to L3 and from the posterior mesenteric ganglion and hypogastric plexus. The source of parasympathetic fibres for ovarium and oviduct are cells of nucleus parasympathicus nervi vagi in the segment located somewhat posteriorly from obex. The source of viscerosensory fibres for the discussed parts of the sexual organs in the cow are cells of the spinal ganglions in the segment Th9 - Th13 and cells of nucleus tractus spinocerebellaris dorsalis localized in neuromers L2 and L3.
在这些研究中,使用了4头性成熟的母牛,双侧或单侧切除其卵巢和输卵管。第四头母牛作为实验对照。手术动物存活21天后宰杀。取材包括:脑干、第9胸节至第13胸节以及第1腰节至第4骶节的脊髓、双侧脊髓神经节、双侧第10胸节至第4骶节的交感干,以及神经节和内脏神经丛,如肠系膜前丛、肠系膜间丛、肠系膜后丛、腹下丛和盆丛。材料用酒精固定,石蜡包埋,切成15微米厚的切片。切片用尼氏法染色。为进行检查,制作了后续切片。由于所做的手术,中枢和外周神经系统的神经细胞发生了退行性变化。这些变化是确定卵巢和输卵管神经中枢定位的基础。这些器官的节前交感内脏运动纤维的来源是第2腰节至第3腰节的中间外侧核。交感节后纤维起源于第1腰节至第3腰节交感干的细胞以及肠系膜后神经节和腹下丛的细胞。卵巢和输卵管的副交感纤维来源是位于闩后方某节段的迷走神经副交感核的细胞。母牛性器官上述部位的内脏感觉纤维来源是第9胸节至第13胸节脊髓神经节的细胞以及位于第2节段和第3节段神经节的脊髓小脑背束核的细胞。