Pande U, Pant H C
Physiol Chem Phys Med NMR. 1984;16(6):463-7.
A metallochromic indicator dye Arsenazo III (Az) was employed to study the effect of ethanol on calcium metabolism in rat erythrocyte ghosts. Addition of 10-150 mM ethanol to a ghost suspension induced a concentration dependent increase in calcium-Arsenazo III (Ca-Az) complex absorbance. Similar results were obtained when ghost suspensions contained (1-10 mM) theophylline. The effect of both, ethanol and theophylline was not observed if the ghosts were pre-treated with 50 microM dantrolene. Theophylline has been reported to stimulate the intracellular release of calcium in a number of cell types and the drug dantrolene is known to inhibit the intracellular release of calcium from the skeletal muscle and nerve cells. These results suggest that ethanol may release calcium from intracellular sites in these ghosts.
采用金属变色指示剂染料偶氮胂III（Az）研究乙醇对大鼠红细胞血影钙代谢的影响。向血影悬液中添加10 - 150 mM乙醇会导致钙 - 偶氮胂III（Ca - Az）复合物吸光度呈浓度依赖性增加。当血影悬液中含有（1 - 10 mM）茶碱时，也得到了类似的结果。如果血影先用50 microM丹曲林预处理，则未观察到乙醇和茶碱的作用。据报道，茶碱可刺激多种细胞类型的细胞内钙释放，而药物丹曲林已知可抑制骨骼肌和神经细胞的细胞内钙释放。这些结果表明，乙醇可能从这些血影的细胞内位点释放钙。
