Pant H C, Virmani M, Majchrowicz E
Subst Alcohol Actions Misuse. 1982;3(6):343-51.
The effects of ethanol on phosphorylation in the erythrocyte ghost membranes was studied. Four groups of rats were used: controls; prodromal phase; overt withdrawal syndrome; and treated with a single dose (6g/kg) of ethanol. Spectrin (Band 2) phosphorylation was enhanced during prodromal phase (200 +/- 12%) and during overt ethanol withdrawal syndrome (150 +/- 18%), while a single dose of ethanol produced no significant change. Addition of micromolar calcium chloride solution to the phosphorylating medium produced pronounced decrease in spectrin phosphorylation in the ghost membranes isolated from controls and those treated with a single dose of ethanol, while this effect of calcium was less pronounced in the ghost membranes isolated from rats in the prodromal phase and in the withdrawal syndrome. This suggests that due to prolonged ethanol treatment membrane phosphorylating properties were less sensitive to the change in calcium concentration. The membrane polypeptide composition was unaffected upon ethanol treatment.
研究了乙醇对红细胞血影膜磷酸化的影响。使用了四组大鼠：对照组；前驱期；明显戒断综合征组；以及用单剂量（6克/千克）乙醇处理的组。血影蛋白（带2）磷酸化在前驱期（200±12%）和明显乙醇戒断综合征期（150±18%）增强，而单剂量乙醇处理未产生显著变化。向磷酸化介质中添加微摩尔氯化钙溶液，使从对照组和单剂量乙醇处理组分离的血影膜中的血影蛋白磷酸化显著降低，而在从前驱期和戒断综合征期大鼠分离的血影膜中，钙的这种作用不太明显。这表明，由于长期乙醇处理，膜的磷酸化特性对钙浓度变化的敏感性降低。乙醇处理对膜多肽组成无影响。
