Burkman L J
Arch Androl. 1984;13(2-3):153-65. doi: 10.3109/01485018408987514.
Suspensions of capacitating human spermatozoa were analyzed for potential hyperactivated movements using videomicrographic methods. Analysis was carried out on aliquots of 22 sperm suspensions, which were proved fertile several hours later during human in vitro fertilization. After approximately 3 h of capacitation, 22.1% of the fertile spermatozoa displayed motility patterns designated as hyperactivated. Over 80% of these hyperactivated spermatozoa moved with a wide-amplitude, two-dimensional whiplash pattern, displaying marked lateral displacement of the head. Only 8.4% of capacitating spermatozoa from oligozoospermic patients showed these hyperactivated movements. The incidence of hyperactivated movements by fertile and oligozoospermic spermatozoa could be significantly increased after exposure to various motility stimulants. The clinical significance of hyperactivation as a functional assay of fertilizing capacity is discussed.
利用视频显微镜方法分析了获能的人类精子悬液的潜在超激活运动。对22份精子悬液的等分试样进行了分析，这些试样在数小时后的人类体外受精过程中被证明具有生育能力。在获能约3小时后，22.1%的有生育能力的精子表现出被指定为超激活的运动模式。这些超激活精子中超过80%以宽幅度的二维鞭打模式运动，头部有明显的侧向位移。少精子症患者的获能精子中只有8.4%表现出这些超激活运动。在暴露于各种运动刺激剂后，有生育能力和少精子症精子的超激活运动发生率可显著增加。讨论了超激活作为受精能力功能检测的临床意义。
