Yanagida T, Nakase M, Nishiyama K, Oosawa F
Nature. 1984;307(5946):58-60. doi: 10.1038/307058a0.
Actin is found in almost all kinds of non-muscle cells where it is thought to have an important role in cell motility. A proper understanding of that role will only be possible when reliable in vitro systems are available for investigating the interaction of cellular actin and myosin. A start has been made on several systems, most recently by Sheetz and Spudich who demonstrated unidirectional movement of HMM-coated beads along F-actin cables on arrays of chloroplasts exposed by dissection of a Nitella cell. As an alternative approach, we report here the direct observation by fluorescence microscopy of the movements of single F-actin filaments interacting with soluble myosin fragments energized by Mg2+-ATP.
肌动蛋白存在于几乎所有类型的非肌肉细胞中，据信它在细胞运动中起重要作用。只有当有可靠的体外系统可用于研究细胞肌动蛋白和肌球蛋白的相互作用时，才有可能正确理解这一作用。已经在几个系统上展开了研究，最近Sheetz和Spudich进行了相关研究，他们展示了包被重链肌球蛋白的珠子沿着通过解剖丽藻细胞而暴露的叶绿体阵列上的F-肌动蛋白丝束进行单向运动。作为一种替代方法，我们在此报告通过荧光显微镜直接观察单个F-肌动蛋白丝与由Mg2 + -ATP提供能量的可溶性肌球蛋白片段相互作用时的运动。
