Bayne M L, Alexander R F, Benbow R M
J Mol Biol. 1984 Jan 5;172(1):87-108. doi: 10.1016/0022-2836(84)90416-9.
A soluble extract of Xenopus laevis ovaries catalyzed ATP-dependent concatenation of linear duplex DNA molecules. DNA ligase and a unique X. laevis DNA binding protein were required for the formation of concatemers. A linear DNA concatenation system was reconstituted using T4 DNA ligase and homogeneous X. laevis DNA binding protein. This system catalyzed intermolecular ligation of DNA molecules into linear concatemers of up to ten or more times monomer length.
非洲爪蟾卵巢的一种可溶性提取物催化线性双链DNA分子的ATP依赖性串联。连接体的形成需要DNA连接酶和一种独特的非洲爪蟾DNA结合蛋白。使用T4 DNA连接酶和纯化的非洲爪蟾DNA结合蛋白重建了一个线性DNA串联系统。该系统催化DNA分子的分子间连接，形成长达单体长度十倍或更长的线性连接体。
