Rusche J R, Howard-Flanders P
Nucleic Acids Res. 1985 Mar 25;13(6):1997-2008. doi: 10.1093/nar/13.6.1997.
Hexamine cobalt chloride (HCC) increases the efficiency of blunt end ligation by T4 DNA ligase about 50 fold. Maximum stimulation occurs when standard buffers for ligation are supplemented with 1 mM HCC. All the ligation events are intermolecular regardless of the initial DNA concentration. In the presence of monovalent cations (eg. 25 mM KCl) HCC still increases the extent of T4 catalyzed ligation but intramolecular ligation products are also formed. Therefore, intermolecular ligation can be performed rapidly and at low DNA concentrations.
六氨合氯化钴（HCC）可使T4 DNA连接酶的平端连接效率提高约50倍。当用于连接的标准缓冲液中添加1 mM HCC时，刺激作用达到最大。无论初始DNA浓度如何，所有连接事件均为分子间连接。在单价阳离子（例如25 mM KCl）存在的情况下，HCC仍会增加T4催化的连接程度，但也会形成分子内连接产物。因此，可以在低DNA浓度下快速进行分子间连接。
