Bishop M C, Ross B D
Transplantation. 1978 May;25(5):235-9. doi: 10.1097/00007890-197805000-00002.
The isolated perfused rat kidney has been used to study the effects of cold ischaemia during ice storage. Tissue adenine nucleotide levels in freeze-clamped kidneys were closely correlated with their function after circulation had been reestablished in the perfusion circuit. The ATP content was depleted to a greater extent than total adenine nucleotide after 8 hr of cold ischaemia but both were considerably diminished after 24 hr. The model was also used to compare four solutions which are available clinically for preliminary flushing of organs in the ice storage preservation technique. The results indicated that for periods of 8 and 24 hr of cold ischaemia, renal function and adenine nucleotide content were significantly better maintained with a solution based on hyperosmolar citrate than with Collins, Sacks, or Perfudex solutions. This study confirmed recent observations of the clinical efficacy of citrate solution, while demonstrating how the isolated perfused rat kidney could be used for rapid screening of modifications in preservation techniques.
离体灌注大鼠肾脏已被用于研究冰存期间冷缺血的影响。在灌注回路中恢复循环后，冷冻钳夹肾脏中的组织腺嘌呤核苷酸水平与其功能密切相关。冷缺血8小时后，ATP含量比总腺嘌呤核苷酸的消耗程度更大，但24小时后两者均显著减少。该模型还用于比较临床上可用于冰存保存技术中器官初步冲洗的四种溶液。结果表明，在冷缺血8小时和24小时期间，与柯林斯溶液、萨克斯溶液或灌注液相比，基于高渗柠檬酸盐的溶液能显著更好地维持肾功能和腺嘌呤核苷酸含量。本研究证实了最近关于柠檬酸盐溶液临床疗效的观察结果，同时展示了如何利用离体灌注大鼠肾脏快速筛选保存技术的改进。
