Jablonski P, Howden B, Marshall V, Scott D
Transplantation. 1980 Oct;30(4):239-43. doi: 10.1097/00007890-198010000-00001.
The isolated rat kidney perfused at 37 C with dialyzed bovine serum albumin (6.5 g/100 ml) in Krebs-Henseleit buffer was used to examine why a hypertonic citrate flush permits rapid recovery of renal function after storage. The composition of the original hyperosmolar citrate solution was varied so that the roles of osmolality, magnesium, and citrate could be evaluated. All kidneys were flushed with the test solutions and stored for 24 hr in the test solutions at 0 C. The citrate flushing solution requires both the citrate anion and magnesium for efficacy. Hyperosmolality does not enhance its action, an isosmolar solution is more effective. Citrate can be replaced by a nonmetabolizable analogue, tricarballylate, if the solution is suitably buffered. The mechanism of action of citrate is still uncertain, it does not seem primarily to act as a metabolic fuel or inhibitor.
将分离的大鼠肾脏在37℃下用Krebs - Henseleit缓冲液中的透析牛血清白蛋白（6.5克/100毫升）进行灌注，用于研究为什么高渗柠檬酸盐冲洗能使肾脏在储存后迅速恢复功能。改变原始高渗柠檬酸盐溶液的成分，以便评估渗透压、镁和柠檬酸盐的作用。所有肾脏均用测试溶液冲洗，并在0℃的测试溶液中储存24小时。柠檬酸盐冲洗溶液的有效性需要柠檬酸盐阴离子和镁。高渗并不能增强其作用，等渗溶液更有效。如果溶液得到适当缓冲，柠檬酸盐可以被不可代谢的类似物三羧基丁酸盐替代。柠檬酸盐的作用机制仍不确定，它似乎主要不是作为代谢燃料或抑制剂起作用。
