Dreikorn K, Horsch R, Röhl L
Eur Urol. 1980;6(4):221-4. doi: 10.1159/000473336.
In 1976 a standardised hypertonic 'intracellular' perfusate (Euro-Collins solution) was recommended for renal preservation in all donor centres collaborating with the Eurotransplant Organisation. In order to investigate the effectiveness of this perfusate canine kidneys were initially flushed with Euro-Collins solution and subjected to 48, 72 and 96 h of cold storage and autotransplantated. Immediate contralateral nephrectomy was performed after autotransplantation. The serum creatinine served as a parameter of postoperative renal function. Under laboratory conditions successful preservation was achieved after minimal (less than 5 min) warm ischaemia for up to 96 h of cold ischaemia and after 15 min of warm ischaemia for up to 48 h of cold ischaemia. Our results suggest that initial flushing and hypothermic storage of kidneys using Euro-Collins solution is a satisfactory preservation method when human cadaver kidneys can be harvested under adequate conditions.
1976年，一种标准化的高渗“细胞内”灌注液（欧洲柯林斯溶液）被推荐给所有与欧洲移植组织合作的供肾中心用于肾脏保存。为了研究这种灌注液的有效性，最初用欧洲柯林斯溶液冲洗犬肾，并进行48、72和96小时的冷藏，然后进行自体移植。自体移植后立即进行对侧肾切除术。血清肌酐作为术后肾功能的参数。在实验室条件下，最短（少于5分钟）热缺血时间下，冷缺血96小时以及15分钟热缺血时间下，冷缺血48小时后均成功实现了保存。我们的结果表明，当能在适当条件下获取人类尸体肾脏时，使用欧洲柯林斯溶液对肾脏进行初始冲洗和低温保存是一种令人满意的保存方法。
