Robinson J
Thromb Res. 1984 Jan 15;33(2):155-62. doi: 10.1016/0049-3848(84)90176-2.
The human diploid fibroblast cell line, MRC-5, derived from embryo lung tissue produced only small quantities of plasminogen activator (PA) when harvested using a standard nutrient medium (Eagle's Minimal Essential Medium, MEM). Use of a schedule designed to induce high concentrations of fibroblast interferon in these cells also resulted in production of considerably enhanced levels of PA. The kinetics of PA production differed from those of interferon production; specifically, PA was produced for at least 6 days following induction despite the toxicity of the inducers whereas interferon synthesis continued for only 1 day. Further investigation of the induction conditions for PA revealed that double-stranded RNA which was absolutely required for interferon production was not required for induction of PA. Indeed, the stimulus for enhancement of PA production appeared to be solely an elevated concentration of calcium ions in the extracellular medium. The possible physiological relevance of this induction of PA by elevated concentrations of calcium ions is discussed.
人二倍体成纤维细胞系MRC - 5源自胚胎肺组织，当使用标准营养培养基（伊格尔最低限度基本培养基，MEM）收获时，仅产生少量纤溶酶原激活物（PA）。使用旨在诱导这些细胞中高浓度成纤维细胞干扰素的方案，也导致PA水平显著提高。PA产生的动力学与干扰素产生的动力学不同；具体而言，尽管诱导剂有毒，但诱导后PA至少产生6天，而干扰素合成仅持续1天。对PA诱导条件的进一步研究表明，干扰素产生绝对需要的双链RNA并非PA诱导所必需。实际上，PA产生增强的刺激似乎仅仅是细胞外培养基中钙离子浓度的升高。讨论了钙离子浓度升高诱导PA的可能生理相关性。
