Couble P, Blaes N, Prudhomme J C
Exp Cell Res. 1984 Apr;151(2):322-31. doi: 10.1016/0014-4827(84)90383-5.
Bombyx mori posterior silkgland cells exhibit an impressive microfilament apparatus located at the cellular apex. It consists of bundles of packed, long microfilaments of 50-70 A diameter running along circumferences delimiting the lumen of the gland, perpendicularly to the flow of luminal silk. Microfilaments are closely associated with microtubules of the cytoplasmic 'radial microtubule system'. Immunolabelling with purified antihuman actin antibodies was used to demonstrate their actin-like nature. Apical microfilaments are sensitive to cytochalasin B (CB) which selectively inhibits the secretion of fibroin. Following the removal of the drug, microfilaments recover their normal morphology and secretion resumes. The possible implication of contraction of microfilaments in the process of secretion is discussed.
家蚕后部丝腺细胞在细胞顶端呈现出令人印象深刻的微丝装置。它由成束的紧密排列的、直径为50 - 70埃的长微丝组成，这些微丝沿着界定腺腔的圆周排列，垂直于腔内丝的流动方向。微丝与细胞质“径向微管系统”的微管紧密相连。使用纯化的抗人肌动蛋白抗体进行免疫标记以证明它们类似肌动蛋白的性质。顶端微丝对细胞松弛素B（CB）敏感，CB可选择性抑制丝心蛋白的分泌。去除药物后，微丝恢复其正常形态，分泌恢复。文中讨论了微丝收缩在分泌过程中的可能作用。
