Kimmel C B, Spray D C, Bennett M V
Dev Biol. 1984 Apr;102(2):483-7. doi: 10.1016/0012-1606(84)90213-6.
During cleavage and blastula stages of embryos of the teleost Fundulus heteroclitus all of the cells are both electotonically coupled and dye coupled to one another, as determined by microelectrode impalements and spread of Lucifer Yellow. At about the time that gastrulation begins we observed a specific loss of junctional coupling between the yolk cell and cells of the blastoderm. Passage of Lucifer Yellow between the yolk cell and blastoderm was reduced at stage 12 (late blastula), and not detected at stage 13 and thereafter, although cells of the blastoderm remain dye coupled to one another through gastrula stages. Also, junctional electrical coupling between the yolk cell and blastoderm became substantially reduced at stage 13 and thereafter. The loss of coupling at this specific cell apposition and time and the large size of the yolk cell may prove useful in analyzing the underlying cellular mechanisms.
在底鳉胚胎的卵裂期和囊胚期，通过微电极刺入和荧光黄扩散测定，所有细胞之间均存在电突触耦合和染料耦合。在原肠胚形成开始时，我们观察到卵黄细胞与胚盘细胞之间的连接耦合出现了特异性丧失。在第12阶段（晚期囊胚），荧光黄在卵黄细胞和胚盘之间的传递减少，在第13阶段及之后未检测到传递，尽管胚盘细胞在原肠胚阶段仍彼此保持染料耦合。此外，卵黄细胞与胚盘之间的连接电耦合在第13阶段及之后大幅减少。在这个特定的细胞附着点和时间出现的耦合丧失以及卵黄细胞的巨大尺寸，可能有助于分析潜在的细胞机制。
