Sambuco C P, Forbes P D
Food Chem Toxicol. 1984 Mar;22(3):233-6. doi: 10.1016/0278-6915(84)90133-9.
For determining threshold levels of inflammation resulting from a phototoxic regimen, measurement of ear thickness with a hand-held micrometer was compared with assessment by eye of the reaction on the dorsa of hairless mice. The ear-measurement technique was less sensitive with respect to the minimum concentration of 8-methoxypsoralen at which phototoxicity could be detected. However this technique was more quantifiable and more objective than detection of threshold responses by eye. Changes in ear thickness were detectable over an extended period of time with a maximum increase at 16 days; return to normal thickness occurred by 35 days. Compressibility of affected ears changed during the course of measurement, but repetitive measurement of the ears did not alter the development or resolution of the inflammatory response.
为确定光毒性治疗方案所导致的炎症阈值水平，使用手持测微计测量耳部厚度，并与通过肉眼评估无毛小鼠背部的反应进行了比较。就可检测到光毒性的8-甲氧基补骨脂素的最低浓度而言，耳部测量技术的敏感性较低。然而，与通过肉眼检测阈值反应相比，该技术更具可量化性和客观性。在较长一段时间内均可检测到耳部厚度的变化，在第16天增加到最大值；到第35天恢复到正常厚度。在测量过程中，受影响耳朵的可压缩性发生了变化，但对耳朵的重复测量并未改变炎症反应的发展或消退情况。
