Edwards M C, Blakeman J P
J Microsc. 1984 Feb;133(Pt 2):205-12. doi: 10.1111/j.1365-2818.1984.tb00486.x.
A method is described for the study of nutrient competition between leaf surface microorganisms and plant pathogenic fungi. Autoradiography was used to investigate the uptake of water-soluble 14C-labelled nutrients by applying a dry pre-formed layer of emulsion to the specimen. The method was adapted for use on both leaf and artificial surfaces. A photometric method was used for rapid examination of autoradiographs.
本文描述了一种研究叶片表面微生物与植物病原真菌之间营养竞争的方法。通过将干燥的预制乳剂层应用于标本,利用放射自显影技术来研究水溶性14C标记营养物质的吸收情况。该方法适用于叶片和人工表面。采用光度法对放射自显影片进行快速检测。