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一种用于确定叶附生植物与植物病原体之间养分竞争的放射自显影方法。

An autoradiographic method for determining nutrient competition between leaf epiphytes and plant pathogens.

作者信息

Edwards M C, Blakeman J P

出版信息

J Microsc. 1984 Feb;133(Pt 2):205-12. doi: 10.1111/j.1365-2818.1984.tb00486.x.

DOI:10.1111/j.1365-2818.1984.tb00486.x
PMID:6538601
Abstract

A method is described for the study of nutrient competition between leaf surface microorganisms and plant pathogenic fungi. Autoradiography was used to investigate the uptake of water-soluble 14C-labelled nutrients by applying a dry pre-formed layer of emulsion to the specimen. The method was adapted for use on both leaf and artificial surfaces. A photometric method was used for rapid examination of autoradiographs.

摘要

本文描述了一种研究叶片表面微生物与植物病原真菌之间营养竞争的方法。通过将干燥的预制乳剂层应用于标本,利用放射自显影技术来研究水溶性14C标记营养物质的吸收情况。该方法适用于叶片和人工表面。采用光度法对放射自显影片进行快速检测。

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引用本文的文献

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Application of microautoradiography to the study of substrate uptake by filamentous microorganisms in activated sludge.应用微量放射自显影技术研究活性污泥丝状微生物对基质的摄取。
Appl Environ Microbiol. 1997 Sep;63(9):3662-8. doi: 10.1128/aem.63.9.3662-3668.1997.
2
Coexistence among Epiphytic Bacterial Populations Mediated through Nutritional Resource Partitioning.通过营养资源分配介导的附生细菌种群共存。
Appl Environ Microbiol. 1994 Dec;60(12):4468-77. doi: 10.1128/aem.60.12.4468-4477.1994.