Zabbo A, Montie J E, Budd G T, Livingston R B
Urology. 1984 Apr;23(4):370-3. doi: 10.1016/0090-4295(84)90143-2.
Recently, techniques have been developed that allow in vitro growth and chemosensitivity testing of human malignancies. We report our initial experience with the clonogenic, or colony-forming, assay as applied to genitourinary malignancies. Over the past two years, 172 specimens of genitourinary tumors were sent for assay. Thirty-three per cent were not plated, either because of insufficient quantity of cells obtained in the sample or because of the low viability percentage as determined by trypan blue exclusion. Forty-seven per cent showed adequate growth for chemosensitivity testing whereas 20 per cent of the samples showed inadequate growth. A major problem was obtaining adequate numbers of viable cells. Several other problems make it difficult to draw clinical correlations from in vitro findings. The method appears promising for several potential applications, but further studies will be needed before the final utility of the methods is defined.
最近，已经开发出了能够对人类恶性肿瘤进行体外培养和化学敏感性测试的技术。我们报告了将克隆形成试验应用于泌尿生殖系统恶性肿瘤的初步经验。在过去两年中，共送检了172份泌尿生殖系统肿瘤标本进行检测。33%的标本未进行接种，原因要么是样本中获得的细胞数量不足，要么是经台盼蓝排斥法测定的活力百分比过低。47%的标本显示出足以进行化学敏感性测试的生长情况，而20%的样本生长不足。一个主要问题是获得足够数量的活细胞。其他几个问题也使得难以从体外研究结果得出临床相关性。该方法在几个潜在应用方面似乎很有前景，但在确定该方法的最终效用之前，还需要进一步研究。
