Waddell W J, Marlowe C, Friedman M A
Food Chem Toxicol. 1984 Apr;22(4):293-303. doi: 10.1016/0278-6915(84)90009-7.
The distribution of [14C]caprolactam was studied by whole-body autoradiography in male, female and 14.5-day-pregnant mice. This technique does not allow translocation or removal of soluble compounds from the sites of localization. Pregnant mice were frozen 20 min and 1, 3, 9 and 24 hr after oral administration of the compound. The non-pregnant mouse was frozen 3 hr after oral dosing; two male mice were frozen 20 min and 9 hr after intravenous administration. Radioactivity was rapidly absorbed from the stomach and distributed throughout the entire animal, including the foetuses. There was efficient elimination by the kidney and liver. Material secreted by the liver into bile and intestinal contents appeared not to be reabsorbed via the enterohepatic circulation. The kinetics of distribution and elimination appeared to be the same in male, female and pregnant animals. The only sites of retention of radioactivity after 24 hr were the umbilical cords, amnion, yolk sac, maternal lens, maternal Harderian gland and maternal liver. The distribution into and removal from the foetuses was typical of molecules that diffuse freely across the placenta. There was no retention of radioactivity in any foetal tissue. With the possible exception of some residual activity in the nasal epithelium, no localization was seen that would suggest a site of toxic action of caprolactam.
通过全身放射自显影技术研究了雄性、雌性以及怀孕14.5天的小鼠体内[14C]己内酰胺的分布情况。该技术不允许可溶性化合物从定位部位发生转运或清除。怀孕小鼠在口服该化合物后20分钟、1小时、3小时、9小时和24小时被冷冻。未怀孕小鼠在口服给药后3小时被冷冻；两只雄性小鼠在静脉注射后20分钟和9小时被冷冻。放射性物质迅速从胃部吸收并分布于整个动物体，包括胎儿。肾脏和肝脏能有效清除该物质。肝脏分泌到胆汁和肠道内容物中的物质似乎不会通过肠肝循环被重新吸收。雄性、雌性和怀孕动物体内的分布和清除动力学似乎相同。24小时后放射性物质的唯一保留部位是脐带、羊膜、卵黄囊、母体晶状体、母体哈德氏腺和母体肝脏。进入胎儿体内和从胎儿体内清除的情况是分子自由扩散穿过胎盘的典型表现。胎儿组织中没有放射性物质的保留。除了鼻上皮中可能存在的一些残留活性外，未观察到表明己内酰胺有毒作用部位的定位情况。
