Ladin B F, Doyle J J, Beachy R N
J Mol Appl Genet. 1984;2(4):372-80.
A naturally occurring variety of soybean Glycine max cv. Keburi, which lacks the alpha'-subunits of the 7S seed storage protein (beta-conglycinin), was recently described by Kitamura and Kaizuma. Keburi beta-conglycinins contain a normal complement of alpha-, beta-, and gamma-subunits that accumulate in a temporally and spatially regulated manner comparable to that of the standard cultivar Provar. Poly(A)+ RNA isolated from mid-to-late maturation stage Keburi seeds was translated in vitro, yielding products equivalent to those of Provar poly(A)+ RNA except that Keburi mRNA did not produce the pre-alpha'-subunit polypeptide. The basis of the Keburi phenotype was determined by examining genomic DNA using Southern blot hybridization. Restriction fragments isolated from a cloned 11.5 kb EcoRI fragment of genomic DNA containing a normal alpha'-subunit gene (Gmg 17.1) were used as hybridization probes. Sequences far upstream of the alpha'-subunit gene were present in both Provar and Keburi cultivars. However, hybridization reactions with probes from within the gene demonstrated that a deletion had occurred in Keburi DNA beginning immediately 5' to the alpha'-subunit gene represented on this 11.5 kb fragment. The deletion extends through most of the coding sequences, producing the Keburi phenotype.
一种天然存在的大豆品种——甘氨酸大豆 cv. 凯布里，它缺乏 7S 种子贮藏蛋白（β-伴大豆球蛋白）的α'-亚基，最近被北村和海津马描述。凯布里β-伴大豆球蛋白含有正常的α-、β-和γ-亚基，它们以与标准品种普罗瓦尔相当的时间和空间调节方式积累。从凯布里种子成熟中后期分离的聚腺苷酸加尾（poly(A)+）RNA 在体外进行翻译，产生的产物与普罗瓦尔 poly(A)+ RNA 的产物相当，只是凯布里 mRNA 不产生前α'-亚基多肽。通过使用 Southern 印迹杂交检查基因组 DNA 来确定凯布里表型的基础。从包含正常α'-亚基基因（Gmg 17.1）的基因组 DNA 的克隆 11.5 kb EcoRI 片段中分离的限制性片段用作杂交探针。α'-亚基基因上游很远的序列在普罗瓦尔和凯布里品种中都存在。然而，用来自基因内部的探针进行的杂交反应表明，在凯布里 DNA 中发生了缺失，该缺失从这个 11.5 kb 片段上代表的α'-亚基基因的 5' 端立即开始。缺失延伸穿过大部分编码序列，产生了凯布里表型。
