移码突变阻止大豆胚胎中Kunitz胰蛋白酶抑制剂mRNA的积累。
A frameshift mutation prevents Kunitz trypsin inhibitor mRNA accumulation in soybean embryos.
作者信息
Jofuku K D, Schipper R D, Goldberg R B
机构信息
Department of Biology, University of California, Los Angeles 90024-1606.
出版信息
Plant Cell. 1989 Apr;1(4):427-35. doi: 10.1105/tpc.1.4.427.
Abstract
We investigated the molecular basis of a soybean Kunitz trypsin inhibitor (KTi) gene mutation that prevents the accumulation of Kunitz trypsin inhibitor protein during seed development. We found that mRNA encoding the major Kunitz trypsin inhibitor protein (KTi3 mRNA) is reduced at least 100-fold in null (KTi-) embryos but that KTi3 gene transcriptional activity is similar in Kunitz trypsin inhibitor producing embryos (KTi+) and in KTi- embryos. We sequenced the Kunitz trypsin inhibitor KTi3 gene from both KTi3+ and KTi3- lines and found that these genes differ by only three nucleotides (+481, +486, and +487) within the KTi3 coding region. Alteration of these nucleotides results in a frameshift within the KTi3- gene that causes premature termination during translation. Our results suggest that the KTi3- frameshift mutation results in KTi3- mRNA destabilization and leads to a drastic reduction in KTi3 mRNA prevalence.
摘要
我们研究了大豆Kunitz胰蛋白酶抑制剂（KTi）基因突变的分子基础，该突变阻止了Kunitz胰蛋白酶抑制剂蛋白在种子发育过程中的积累。我们发现，在无（KTi-）胚胎中，编码主要Kunitz胰蛋白酶抑制剂蛋白的mRNA（KTi3 mRNA）减少了至少100倍，但KTi3基因的转录活性在产生Kunitz胰蛋白酶抑制剂的胚胎（KTi+）和KTi-胚胎中相似。我们对来自KTi3+和KTi3-品系的Kunitz胰蛋白酶抑制剂KTi3基因进行了测序，发现这些基因在KTi3编码区内仅相差三个核苷酸（+481、+486和+487）。这些核苷酸的改变导致KTi3-基因内的移码，从而在翻译过程中导致提前终止。我们的结果表明，KTi3-移码突变导致KTi3- mRNA不稳定，并导致KTi3 mRNA丰度急剧降低。
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本文引用的文献
1
Soybean lectin and related proteins in seeds and roots of le and le soybean varieties.le和le大豆品种种子及根中的大豆凝集素及相关蛋白。
Plant Physiol. 1986 Jun;81(2):558-65. doi: 10.1104/pp.81.2.558.
2
Isolation and Characterization of Messenger RNAs for Seed Lectin and Kunitz Trypsin Inhibitor in Soybeans.大豆种子凝集素和库尼兹胰蛋白酶抑制剂信使核糖核酸的分离与特性分析
Plant Physiol. 1981 Sep;68(3):766-71. doi: 10.1104/pp.68.3.766.
3
Cellular localization of soybean storage protein mRNA in transformed tobacco seeds.大豆贮藏蛋白 mRNA 在转化烟草种子中的细胞定位。
Proc Natl Acad Sci U S A. 1988 Jan;85(2):458-62. doi: 10.1073/pnas.85.2.458.
4
Transcriptional and post-transcriptional regulation of soybean seed protein mRNA levels.大豆种子蛋白 mRNA 水平的转录后调控。
Proc Natl Acad Sci U S A. 1986 Apr;83(7):2123-7. doi: 10.1073/pnas.83.7.2123.
5
Molecular analysis of two phytohemagglutinin genes and their expression in Phaseolus vulgaris cv. Pinto, a lectin-deficient cultivar of the bean.两个血凝素基因的分子分析及其在菜豆（Phaseolus vulgaris cv. Pinto），一种缺乏凝集素的菜豆品种中的表达。
EMBO J. 1986 Dec 1;5(12):3075-82. doi: 10.1002/j.1460-2075.1986.tb04613.x.
6
7
Molecular characterization of a deletion mutation affecting the alpha'-subunit of beta-conglycinin of soybean.影响大豆β-伴大豆球蛋白α'-亚基的缺失突变的分子特征分析
J Mol Appl Genet. 1984;2(4):372-80.
8
Ultrastructural localization of Kunitz inhibitor on thin sections of Glycine max (soybean) cv. Maple Arrow by the gold method.采用金标法对大豆品种Maple Arrow的薄切片中库尼兹抑制剂进行超微结构定位。
Histochemistry. 1983;77(1):37-50. doi: 10.1007/BF00496634.
9
mRNA-deficient beta o-thalassemia results from a single nucleotide deletion.mRNA缺陷型β地中海贫血由单个核苷酸缺失引起。
Nucleic Acids Res. 1982 Sep 25;10(18):5421-7. doi: 10.1093/nar/10.18.5421.
10
Structure and flanking regions of soybean seed protein genes.大豆种子蛋白基因的结构及侧翼区域。
Cell. 1982 Jun;29(2):651-60. doi: 10.1016/0092-8674(82)90181-7.
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